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Related Experiment Videos

Accuracy of calibrated-loop transfer.

A C Albers, R D Fletcher

    Journal of Clinical Microbiology
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    The accuracy of a 0.001-milliliter calibrated platinum loop is highly variable. Loop withdrawal angle and container diameter significantly impact sample volume, leading to potential errors exceeding 50%.

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    Area of Science:

    • Analytical Chemistry
    • Laboratory Techniques

    Background:

    • Accurate liquid handling is crucial in scientific research.
    • Calibrated platinum loops are commonly used for precise sample volumes.

    Purpose of the Study:

    • To evaluate the accuracy of a 0.001-ml calibrated platinum loop.
    • To identify factors influencing the delivery volume of the platinum loop.

    Main Methods:

    • Visible absorption spectroscopy was employed to determine loop accuracy.
    • Weight determinations were used as a secondary method for validation.

    Main Results:

    • Loop delivery volume is dependent on withdrawal angle and container diameter.
    • Vertical sampling from narrow containers (≤7-mm ID) yielded ~50% of the target volume.

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  • Sampling at a 45° angle from wide containers (≥22-mm ID) yielded >150% of the target volume, a threefold difference.

    • Conclusions:

    • The 0.001-ml platinum loop exhibits significant inaccuracy due to sampling technique.
    • Variations in withdrawal angle and container size can lead to substantial errors (+/- 50%) in delivered volume.
    • Standardization of sampling procedures is essential for reliable results when using platinum loops.