Differential protein synthesis in Candida albicans during blastospore formation at 24.5 degrees C and during germ tube formation at 37 degrees C

To identify proteins synthesized during blastospore to germ tube transformation in Candida albicans, membrane and cytoplasmic protein fractions labelled with 14C were analysed by SDS-PAGE and autoradiography. Four cytoplasmic proteins were detected in pulse-labelled lysates prepared from cells forming blastospores and germ tubes at 37 degrees C, but not in pulse-labelled lysates prepared from cells forming blastospores at 24.5 degrees C. Three proteins detectable in 24.5 degrees C lysates were diminished in 37 degrees C lysates.