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Interfacing a microcomputer to a spectrophotometer for EMIT assays.

J Morton

    Annals of Clinical Biochemistry
    |July 1, 1983
    PubMed
    Summary
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    This study details connecting a microcomputer to a spectrophotometer for laboratory automation. The developed BASIC subroutines enable automated sample sensing, data transfer, and flowcell purging for enhanced experimental efficiency.

    Area of Science:

    • Analytical Chemistry
    • Laboratory Automation
    • Scientific Instrumentation

    Background:

    • Spectrophotometers are crucial for quantitative analysis in various scientific fields.
    • Manual data handling and sample processing can be time-consuming and prone to errors.
    • Integrating microcomputers offers potential for automating laboratory workflows.

    Purpose of the Study:

    • To describe the connection of a microcomputer to a spectrophotometer.
    • To present BASIC subroutines for automating key laboratory functions.
    • To demonstrate the adaptability of this system for diverse laboratory assays.

    Main Methods:

    • Development of four BASIC subroutines for microcomputer control.
    • Subroutines cover sample input sensing, data transfer, flowcell purging, and time delay loops.

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  • Integration of a microcomputer with a spectrophotometer for real-time data acquisition.

    • Main Results:

    • Successful implementation of a microcomputer-spectrophotometer interface.
    • Demonstrated automation of sample sensing, data transfer, and flowcell purging.
    • The system was initially configured for EMIT (Enzyme Multiplied Immunoassay Technique) assays.

    Conclusions:

    • The described microcomputer-spectrophotometer connection provides a robust platform for laboratory automation.
    • The BASIC subroutines offer a flexible solution for streamlining experimental procedures.
    • This approach is readily adaptable to various laboratory tests beyond EMIT assays, enhancing overall research efficiency.