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Updated: Aug 14, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
A 45 000 molecular weight human renin precursor is synthesized in a cell-free translation system
Abstract:
Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]methionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit gamma-globulin antisera. A 15S mRNA has been found to direct synthesis of a 45 000 molecular weight protein immunoprecipitable with anti-human renin. This protein is considered to be the ultimate precursor of renin (pre-prorenin).
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