Coexistence of neuropeptides in hydra

Using a technique for simultaneous visualisation of two antigens in one section, oxytocin-like immunoreactivity has been found to coexist with bombesin-like immunoreactivity in neurons of the basal disk, gastric region and tentacles of hydra. Neurons with oxytocin-like immunoreactivity in peduncle and hypostome, on the other hand, have little or no bombesin-like material. Oxytocin-like immunoreactivity never coexists with FMRFamide-immunoreactivity. The neurons with oxytocin- and FMRFamide-like immunoreactivity, however, are often found to be closely intermingled. The results show that coexistence, as well as non-coexistence, of neuropeptides is a phylogenetically old principle.