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A solid phase C1q enzyme assay for circulating immune complexes adapted for routine clinical laboratory testing.

K James, A Vahey, M Robinson

American Journal of Clinical Pathology

|October 1, 1983

Summary

A new assay for circulating immune complexes (CIC) is available for clinical labs. This solid phase C1q (SP C1q) enzyme immunoassay (EIA) is reproducible and specific for dsDNA-anti-DNA immune complexes.

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Area of Science:

Clinical chemistry
Immunology
Laboratory diagnostics

Background:

Circulating immune complexes (CIC) are biomarkers in various autoimmune diseases.
Accurate and reproducible detection of CIC is crucial for diagnosis and monitoring.
Existing assays may lack adaptability for routine clinical laboratory settings.

Purpose of the Study:

To describe a novel assay for circulating immune complexes (CIC).
To evaluate the adaptability of the assay for routine clinical laboratory use.
To demonstrate the specificity of the assay for clinically relevant immune complexes.

Main Methods:

Development of a solid phase C1q (SP C1q) enzyme immunoassay (EIA).
Utilized commercially available reagents for assay implementation.

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Assessed assay reproducibility using coefficient of variation (CV%).

Main Results:

The SP C1q EIA is adaptable to routine clinical laboratory settings.
The assay demonstrated reproducibility with CV% less than 20% in the abnormal range.
Specificity was confirmed for detecting dsDNA-anti-DNA immune complexes.

Conclusions:

The described SP C1q EIA is a reliable and reproducible method for detecting CIC.
This assay is suitable for routine clinical laboratory implementation.
The assay specifically detects clinically significant dsDNA-anti-DNA immune complexes, aiding in autoimmune disease diagnostics.