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Related Experiment Videos

Opsonization of various capsular (K) E. coli by the alternative complement pathway.

P Stevens, L S Young, S Adamu

    Immunology
    |November 1, 1983
    PubMed
    Summary

    Most Escherichia coli (E. coli) capsular types are effectively opsonized by the alternative complement pathway (ACP). However, E. coli K3, K5, K12, and K92 show poor opsonization by the ACP, suggesting a potential virulence factor.

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    Area of Science:

    • Immunology
    • Microbiology
    • Bacteriology

    Background:

    • Escherichia coli (E. coli) K-1 utilizes its capsule as a virulence factor by evading the alternative complement pathway (ACP).
    • The opsonization capabilities of other E. coli capsular types via the ACP remain largely uncharacterized.

    Purpose of the Study:

    • To investigate the susceptibility of various capsular types of E. coli to opsonization by the alternative complement pathway (ACP).

    Main Methods:

    • Assessed E. coli opsonization using whole blood luminol-dependent chemiluminescence (CL).
    • Blocked the classical complement pathway using magnesium ethyleneglycol tetraacetate to isolate ACP activity.

    Main Results:

    • Most E. coli K-types (6, 7, 27, 30, 42, 53, 57, 75) showed effective opsonization (>65% CL) via the ACP.

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  • K-types 2 and 13 exhibited both high and intermediate opsonization by the ACP.
  • E. coli K-types 1, 3, 5, 12, and 92 were poorly opsonized (<35% CL) by the ACP.

    • Conclusions:

    • The majority of E. coli capsular types are readily opsonized by the alternative complement pathway.
    • Poor opsonization of specific E. coli K-types (K3, K5, K12, K92) by the ACP may represent a significant virulence mechanism.