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Human skin storage techniques: a study utilizing a nude mouse recipient.

A E Cram, M Domayer, J Shelby

    The Journal of Trauma
    |October 1, 1983
    PubMed
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    Storing human skin for transplantation is feasible for 20 days using Roswell Park Memorial Institute (RPMI) medium. Low antibiotic concentrations enhance skin viability, while fetal calf serum offers no benefit.

    Area of Science:

    • Biomedical research
    • Tissue engineering
    • Transplantation science

    Background:

    • The viability of stored human skin is critical for successful transplantation.
    • Nude mouse models offer a viable platform for assessing skin storage conditions.

    Purpose of the Study:

    • To evaluate the efficacy of Roswell Park Memorial Institute (RPMI) medium for storing viable human skin.
    • To determine the impact of antibiotic concentration and fetal calf serum on skin viability during storage.

    Main Methods:

    • Human skin samples were stored in RPMI medium for up to 20 days.
    • Different concentrations of penicillin and streptomycin were tested.
    • The effect of adding fetal calf serum to the storage medium was assessed.
    • Skin viability was evaluated using the nude mouse model.

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    Main Results:

    • Human skin remained viable for 20 days when stored in RPMI medium.
    • Lower concentrations of penicillin and streptomycin provided adequate bacterial control and improved skin viability compared to higher concentrations.
    • The addition of fetal calf serum did not enhance human skin viability.

    Conclusions:

    • RPMI medium supports the reliable storage of viable human skin for 20 days.
    • Optimizing antibiotic concentrations is crucial for maintaining skin viability during storage.
    • Fetal calf serum is not essential for preserving human skin viability in this storage model.