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Related Experiment Videos

A plasmid-linked caseinolytic activity in Enterobacteriaceae.

P Mignatti, L Pagani, E Romero

    Microbiologica
    |July 1, 1983
    PubMed
    Summary

    Certain R factors from Klebsiella pneumoniae confer caseinolytic activity in E. coli. This activity may be regulated by plasmid-linked genes, with an inhibitor present in E. coli K12 J62.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • The study investigates caseinolytic activity, an enzyme that breaks down casein.
    • Escherichia coli (E. coli) K12 strains are common models for bacterial research.
    • R factors, or resistance plasmids, carry genes conferring antibiotic resistance.

    Purpose of the Study:

    • To determine if R factors from clinical isolates can confer caseinolytic activity to E. coli K12.
    • To characterize the nature of this caseinolytic activity and its genetic basis.
    • To explore the potential role of plasmid-linked regulatory genes.

    Main Methods:

    • Testing E. coli K12 J62 strains carrying various R factors for caseinolytic activity.
    • Analyzing R factors from clinical Enterobacteriaceae isolates.
    • Phenotypic characterization of antibiotic resistance and lactose fermentation (lac+).
    • Investigating the presence of caseinolytic inhibitors in E. coli strains.

    Main Results:

    • Caseinolytic activity (cas+) was detected in 7 out of 139 tested R factors.
    • All cas+ R factors originated from Klebsiella pneumoniae and carried lac+ and multiple antibiotic resistance genes (R-lac factors).
    • A plasmid-free E. coli K12 strain (J53) exhibited caseinolytic activity, while strain J62 showed an inhibitory effect on this activity.

    Conclusions:

    • Plasmid-linked genes, specifically from Klebsiella pneumoniae R factors, can confer caseinolytic activity in E. coli.
    • The expression of caseinolytic activity in E. coli may be influenced by plasmid-encoded regulatory elements.
    • E. coli K12 J62 possesses a specific inhibitor of caseinolytic activity found in certain R factors and strains.

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