A cryostat-freeze drying technique for enhanced visualization of catecholamine/indoleamine-containing neurons

A modification of the histofluorescent technique is described which allows for the enhanced visualization of catecholamine/indoleamine (CA/IN)-containing neurons and fibers in CNS and peripheral tissues. Utilizing freeze-dried, cryostat sections of various tissues, the cytoplasmic content of CA/IN-containing vesicles could be localized within the boundaries of identifiable neurons with minimal indication of bioamine dispersion. The present procedure is applicable for the histological assessment of rapid changes in neuronal CA content resulting from experimental manipulations and allows for the simultaneous biochemical measurement of endogenous bioamine content in cells without interference from pharmacological blocking agents.