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Related Experiment Videos

Colloidal gold: a pluripotent receptor probe.

D A Handley, S Chien

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |October 1, 1983
    PubMed
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    Colloidal gold nanoparticles offer versatile labeling for biological molecules. Their electron density and tunable size make them ideal probes for studying cell biology, including receptors and protein functions.

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Nanotechnology

    Background:

    • Colloidal gold is an electron-dense, lyophobic colloid.
    • It forms stable electrostatic interactions with various macromolecules.
    • Monodispersed colloids (3-150 nm) offer researchers flexibility in probe selection.

    Purpose of the Study:

    • To highlight the versatility and applications of colloidal gold as a probe in cell biology.
    • To demonstrate its utility in studying surface antigens, cell components, and receptor-ligand interactions.
    • To showcase its use in investigating enzyme-substrate reactions and transcellular pathways.

    Main Methods:

    • Gold labeling of antibodies and lectins for studying cell surface components.
    • Application of colloidal gold for labeling diverse biomolecules including metabolites, enzymes, hormones, plasma proteins, and toxins.

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  • Utilizing the electron-dense nature of gold for high-contrast visualization in biological systems.

    • Main Results:

    • Extensive use of gold labeling for antibodies and lectins to study surface antigens and cell components.
    • Successful extension of gold labeling to receptor-ligand binding, enzyme-substrate reactions, and transcellular pathways.
    • Demonstrated application in labeling a wide range of molecules: metabolites, enzymes, hormones, plasma proteins, and endotoxins.

    Conclusions:

    • Colloidal gold is a versatile and useful probe in cell biology.
    • Its applications span the study of receptors, endocytosis, transport, and protein functions.
    • The tunable size and electron density of colloidal gold facilitate diverse biological investigations.