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In vitro-produced antibody-coated bacteria.

N Fujimura, M Yuasa, M Ghazizadeh

    Urologia Internationalis
    |January 1, 1983
    PubMed
    Summary

    A novel in vitro method identifies ACB-positive bacteria in polymicrobial renal bacteriuria. These bacteria, often the most abundant, may impair kidney function, suggesting a need for targeted eradication.

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    Area of Science:

    • Microbiology
    • Nephrology
    • Infectious Diseases

    Background:

    • Polymicrobial renal bacteriuria is common in patients with permanent nephrostomies.
    • Asymptomatic bacteriuria can indicate underlying renal issues.
    • Identifying specific bacterial roles in complex urinary tract infections is challenging.

    Purpose of the Study:

    • To develop and validate a new in vitro method for isolating and identifying ACB-positive bacteria in polymicrobial renal bacteriuria.
    • To assess the prevalence of ACB-positive bacteria in patients with nephrostomies and asymptomatic bacteriuria.
    • To investigate the correlation between ACB-positive bacteria and renal function decline.

    Main Methods:

    • Development of a novel in vitro diagnostic method for ACB-positive bacteria detection.
    • Application of the method to urine samples from 16 patients with permanent nephrostomies and asymptomatic bacteriuria.
    • Analysis of 48 urinary isolates, with identification and ACB-positivity assessment.
    • Correlation of ACB-positive bacterial species with colony counts and patient renal function (creatinine clearance).

    Main Results:

    • The new method identified ACB-positive bacteria in 21 out of 48 (43.8%) isolates from 16 patients.
    • In most infected patients (13/16), only one bacterial species tested positive for ACB.
    • ACB-positive bacteria were predominantly found in species with high colony counts (>10(5)/ml), present in 71.5% of cases.
    • A significant decline in renal function was observed in 11 out of 13 patients with available creatinine clearance data.

    Conclusions:

    • The developed in vitro method effectively isolates and identifies ACB-positive bacteria in polymicrobial renal bacteriuria.
    • ACB-positive bacterial species, particularly those with high counts, may play an aggressive role in polymicrobial urinary tract infections.
    • The findings suggest a potential link between ACB-positive bacteria and decreased renal function, highlighting the need for their eradication.

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