Related Experiment Videos
A technique for the concentration of nucleated bone marrow cells for in vitro manipulation or cryopreservation using
Abstract:
27 bone marrow aspirates were processed on the IBM 2991 Blood Cell Processor to achieve a reduction in volume (greater than 80%) and in red blood cell contamination (greater than 75%), without loss (less than 20%) of nucleated cells. The procedure was used to concentrate nucleated bone marrow cells either prior to cryopreservation for subsequent autologous transplantation, or prior to incubation with a murine monoclonal antibody (OKT3) for allogeneic transplantation. We conclude that the procedures used for the concentration, cryopreservation, thawing and infusion do not adversely affect the viability of cells as assessed by in vitro culture (CFU-GM). Of the marrows processed, 12 have been reinfused and resulted in successful engraftment.
Insights
Bone marrow processing using the IBM 2991 Blood Cell Processor effectively concentrates nucleated cells for transplantation. Procedures maintain cell viability, leading to successful engraftment in patients undergoing autologous or allogeneic transplants.
Area of Science:
- Hematology
- Cellular Biology
- Transplantation Medicine
Background:
- Bone marrow processing is crucial for cell transplantation.
- Efficient concentration and preservation of nucleated cells are vital for successful engraftment.
- Minimizing red blood cell contamination is a key challenge.
Purpose of the Study:
- To evaluate the efficacy of the IBM 2991 Blood Cell Processor for bone marrow processing.
- To assess the impact of processing on nucleated cell viability and red blood cell contamination.
- To determine the success of transplantation following the described processing methods.
Main Methods:
- Processing of 27 bone marrow aspirates using the IBM 2991 Blood Cell Processor.
- Concentration of nucleated cells, reduction of red blood cell contamination, and volume reduction.
- Assessment of cell viability using in vitro culture (CFU-GM) post-processing, cryopreservation, thawing, and infusion.
Main Results:
- Achieved >80% volume reduction and >75% red blood cell contamination reduction.
- Nucleated cell loss was <20%.
- 12 reinfused marrows resulted in successful engraftment, indicating preserved cell function.
Conclusions:
- The IBM 2991 processor effectively concentrates bone marrow cells while preserving viability.
- Processing, cryopreservation, and thawing do not adversely affect CFU-GM viability.
- The procedure is suitable for preparing bone marrow for autologous and allogeneic transplantation.