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Extracellular proteases from Legionella.

B P Berdal

    Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie = International Journal of Microbiology and Hygiene. A, Medical Microbiology, Infectious
    |July 1, 1983
    PubMed
    Summary
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    I. Sachverzeichnis.

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·2020
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    I. Verzeichnis der in Band 255 enthaltenen Arbeiten.

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·2020
    Same journal

    II. Sachverzeichnis.

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·2020
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    Pathogenicity tests with strains of Edwardsiella tarda: detection of a heat-stable enterotoxin.

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·1983
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    [Some indices of organ reactivity in experimental trichinellosis. II. Lysozyme activity in trichinellosis in rabbits].

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·1983
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    [Some indices of organ reactivity in experimental trichinellosis. I. Granulocyte reactions in trichinellosis in rabbits].

    Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...·1983
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    Synthetic peptides enable specific detection of bacterial endopeptidase activity in Legionella. Hydrolysis patterns reveal species-specific differences, aiding in the identification of Legionella pneumophila strains and differentiating them from other species.

    Area of Science:

    • Microbiology
    • Enzymology
    • Biochemistry

    Background:

    • Bacterial endopeptidases (proteinases) play crucial roles in microbial physiology and pathogenesis.
    • Accurate detection and characterization of these enzymes are vital for bacterial identification and understanding virulence mechanisms.
    • Existing methods for measuring proteolytic activity may have limitations in specificity or scope.

    Purpose of the Study:

    • To introduce and validate a novel method for specifically registering bacterial endopeptidase activity using synthetic chromogenic peptides.
    • To establish and analyze peptide hydrolysis patterns for various Legionella species.
    • To investigate the presence and localization of endopeptidases in different Legionella strains, including extracellular and cell-bound forms.

    Main Methods:

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    • Utilized synthetic chromogenic peptides as substrates to detect bacterial endopeptidase activity.
    • Applied concentrated broth filtrates from Legionella bacteria to a panel of different peptides.
    • Assayed for both extracellular and cell- or cell wall-bound endopeptidase activities across multiple Legionella strains.

    Main Results:

    • The synthetic chromogenic peptide method provides a specific means to register bacterial endopeptidase activity, complementing existing techniques.
    • Distinct peptide hydrolysis patterns were observed for Legionella species, demonstrating a degree of species specificity.
    • A close similarity in hydrolysis patterns was found among Legionella pneumophila strains, differentiating them from other Legionella species. Legionella micdadei strain Tatlock showed no extracellular endopeptidase activity but possessed cell-bound endopeptidases, as did all other tested Legionella strains.

    Conclusions:

    • Synthetic chromogenic peptides offer a valuable tool for the specific detection and characterization of bacterial endopeptidases.
    • Peptide hydrolysis profiling can serve as a useful adjunct for the taxonomic differentiation of Legionella species.
    • Both extracellular and cell-bound endopeptidases are present in Legionella, with varying patterns that can aid in strain and species identification.