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Related Experiment Videos

[TEM-type beta-lactamase coded by the plasmid from Citrobacter sp].

A Iu Sazykin, S M Navashin

    Antibiotiki
    |August 1, 1983
    PubMed
    Summary

    This study characterizes a TEM-type beta-lactamase from E. coli, revealing its enzyme kinetics and structural properties. The enzyme shows specific activity against certain antibiotics and structural stability during methicillin binding.

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    [Contemporary molecular-biological methods of cancer diagnosis and monitoring based on the humoral immune response to tumor-associated antigens].

    Molekuliarnaia genetika, mikrobiologiia i virusologiia·2007

    Area of Science:

    • Microbiology and Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Beta-lactamase enzymes confer bacterial resistance to beta-lactam antibiotics.
    • Understanding beta-lactamase properties is crucial for combating antibiotic resistance.

    Purpose of the Study:

    • To isolate, purify, and characterize a beta-lactamase from E. coli strain 1039.
    • To determine the enzyme's kinetic parameters, substrate profile, and structural features.

    Main Methods:

    • Enzyme isolation and purification from E. coli.
    • Potentiometric assay for enzyme activity determination.
    • Gel filtration for molecular weight determination.
    • Circular dichroism spectroscopy for structural analysis.

    Main Results:

    • Purified beta-lactamase exhibited TEM-type substrate profile and high specific activity.
    • Molecular weight was determined to be 21,500 Da.
    • Methicillin significantly reduced enzyme activity, while other antibiotics did not.
    • Circular dichroism revealed stable alpha- and beta-structural content upon methicillin binding.

    Conclusions:

    • The characterized beta-lactamase is a TEM-type enzyme with specific inhibitory characteristics.
    • The enzyme's active site appears structurally rigid during catalysis, as indicated by CD analysis.
    • Further studies on this enzyme could inform strategies against antibiotic resistance.

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