Related Experiment Video

Updated: Jul 21, 2026

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

The microtrabecular lattice and its relationships to other organelles in prokaryotes and eukaryotes--high resolution

G P Epling, J A Blixt, R W Mahurin

    Cytobios
    |January 1, 1983
    PubMed

    Keywords:
    cell structureelectron microscopyorganelle relationshipsmicrotrabecular latticescanning electron microscopy

    More Related Videos

    Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy09:12

    Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

    Published on: June 9, 2022

    Visualization of Organelles In Situ by Cryo-STEM Tomography08:37

    Visualization of Organelles In Situ by Cryo-STEM Tomography

    Published on: June 23, 2023

    Related Concept Videos

    Studying the Cytoskeleton01:17

    Studying the Cytoskeleton

    The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
    Overview of Electron Microscopy01:25

    Overview of Electron Microscopy

    The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
    Scanning Electron Microscopy01:07

    Scanning Electron Microscopy

    A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
    Fundamental Principles
    Accelerated...
    Electron Microscope Tomography and Single-particle Reconstruction01:07

    Electron Microscope Tomography and Single-particle Reconstruction

    Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
    Electron Tomography
    Electron tomography can be performed either in TEM or STEM (scanning transmission...