Related Experiment Video

Updated: Aug 14, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
10:32

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

A rapid assay for immunoglobulin in hybridoma supernatants

D Male, G Pryce

    Immunological Communications
    |October 1, 1983
    PubMed

    A single step assay is described which allows detection of IgM and IgG in tissue culture supernatants at concentrations below 100 ng/ml. The assay is based on the agglutination of staphylococcal protein A coated sheep red blood cells (E-spA) in the presence of an enhancing antibody. The assay was designed for the rapid screening of antibody production by hybridoma cultures.

    More Related Videos

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology09:42

    Generation of Murine Monoclonal Antibodies by Hybridoma Technology

    Published on: January 2, 2017

    Generation of Monoclonal Antibodies Against Natural Products12:15

    Generation of Monoclonal Antibodies Against Natural Products

    Published on: April 6, 2019

    Related Concept Videos

    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.