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Yeast thermotolerance does not require protein synthesis.

B G Hall

    Journal of Bacteriology
    |December 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Heat shock proteins in yeast are induced by heat but do not cause thermotolerance. Protein synthesis is not required for yeast to develop heat tolerance.

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    Area of Science:

    • Molecular Biology
    • Cellular Stress Response
    • Yeast Genetics

    Background:

    • Heat shock triggers the synthesis of specific stress proteins in Saccharomyces cerevisiae.
    • Simultaneously, heat shock confers acquired thermotolerance, a protective state against subsequent heat stress.
    • The relationship between stress protein synthesis and thermotolerance induction remains incompletely understood.

    Purpose of the Study:

    • To investigate the causal role of stress (heat shock) proteins in the induction of thermotolerance in Saccharomyces cerevisiae.
    • To determine if protein synthesis is a prerequisite for acquiring thermotolerance at 37 degrees C.

    Main Methods:

    • Yeast cultures were subjected to heat shock at 37 degrees C.
    • Amino acid analogs were used to induce stress protein synthesis without heat shock.

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  • Thermotolerance was assessed after these treatments.
  • Protein synthesis inhibition was employed to evaluate its necessity for thermotolerance induction.

    • Main Results:

    • Heat shock at 37 degrees C induced both stress protein synthesis and thermotolerance.
    • Amino acid analogs induced stress protein synthesis but failed to induce thermotolerance.
    • Thermotolerance was induced at 37 degrees C even when protein synthesis was inhibited.

    Conclusions:

    • Stress (heat shock) proteins are not causally involved in the induction of thermotolerance at 37 degrees C in Saccharomyces cerevisiae.
    • Acquired thermotolerance at 37 degrees C can be induced independently of new protein synthesis.