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Evaluation of API microtube GT system for detection of germ tube production by clinically significant yeasts

A Philip, A Tsui, J E O'Brien

    Journal of Clinical Microbiology
    |November 1, 1983
    PubMed

    Insights

    The API GT system is less sensitive for detecting yeast germ tubes compared to conventional methods. Conventional methods are also more cost-effective for yeast identification.

    Area of Science:

    • Medical Mycology
    • Clinical Microbiology

    Background:

    • Accurate identification of yeast isolates is crucial in clinical settings.
    • Germ tube production is a key preliminary test for yeast identification, particularly for *Candida albicans*.
    • Evaluating diagnostic tools for efficiency and cost-effectiveness is essential for laboratory practice.

    Purpose of the Study:

    • To compare the sensitivity of the API GT system with a conventional method for detecting yeast germ tube production.
    • To assess the cost-effectiveness of the API GT system versus the conventional method.

    Main Methods:

    • A total of 152 fresh yeast isolates were tested.
    • Germ tube production was assessed using the API GT system.
    • Germ tube production was also assessed using a conventional slide culture method.

    Main Results:

    • The API GT system demonstrated lower sensitivity in detecting germ tubes compared to the conventional method.
    • The cost per test for the API GT system was $0.76.
    • The cost per test for the conventional method was $0.39.

    Conclusions:

    • The conventional method is more sensitive for germ tube detection than the API GT system.
    • The conventional method offers a significant cost advantage over the API GT system for this diagnostic test.

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