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An ELISA method for quantitation of macrophage migration from agarose microdroplets
Journal of Immunological Methods
|November 11, 1983
Abstract:
A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. The migration is determined with polyclonal anti-macrophage antibodies in an ELISA assay. The numbers of macrophages thus detected correlate well with the migration as measured by a capillary tube assay for MIF.
