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Updated: Sep 21, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
Role of glutathione in affecting the radiosensitivity of molecular and cellular systems
Abstract:
It has previously been shown that radioinduced organic radicals can be repaired by hydrogen donation from glutathione (GSH) and this repair is in competition with oxygen (damage fixation). In this paper the influence of exogenous glutathione on the radiation response of the enzyme alcohol dehydrogenase (YADH), DNA in vitro, and E. coli B/r cells has been investigated. GSH is observed to protect YADH essentially by free radical scavenging mechanisms in both presence or absence of oxygen. The same mechanism seems operate in the radioprotection afforded by GSH to DNA in vitro. E. coli B/r cells are protected at higher extent by GSH than its oxidized form (GSSG); the possibility that GSH penetrate into bacterial cells more easily that GSSG can explain their different behaviour. None of the three systems studied has provided definitive support for the occurrence of the hydrogen donation reaction in the radioprotective mechanisms of GSH versus biomolecules and bacterial cells.
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