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Selective pigment medium for Streptococcus agalactiae.

A C Albers, J M Sniffen, D N Freedel

    The American Journal of Medical Technology
    |November 1, 1983
    PubMed
    Summary

    A new selective pigment medium aids in identifying Streptococcus agalactiae (Group B Streptococcus). This medium is crucial for detecting the bacteria in neonatal samples, improving diagnostics for infant health.

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    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Neonatal Health

    Background:

    • Streptococcus agalactiae (Group B Streptococcus) poses significant risks for neonatal morbidity and mortality.
    • Existing diagnostic methods require improvement for early and accurate detection.

    Purpose of the Study:

    • To develop and evaluate a selective and differential primary plating medium for Streptococcus agalactiae.
    • To enable presumptive identification of S. agalactiae directly from polymicrobic specimens.

    Main Methods:

    • A selective pigment medium containing colistin and nalidixic acid was developed.
    • Preliminary studies assessed pigment production by known S. agalactiae strains across three media.
    • Clinical laboratory trials were conducted on specimens from neonates, female reproductive tracts, and urinary tracts.

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  • An epidemiological investigation was performed on a mother/infant population.

    • Main Results:

    • The selective pigment medium demonstrated effectiveness in identifying S. agalactiae.
    • In a clinical trial, 581 specimens were screened.
    • An epidemiological study screened 1,331 specimens, with 5.6% positive on sheep blood agar and 5.9% on the selective pigment medium.

    Conclusions:

    • The novel selective pigment medium offers a valuable tool for the presumptive identification of Streptococcus agalactiae.
    • This medium can be effectively used in clinical settings for screening various specimen types, including those from neonates.
    • The development supports enhanced diagnostics for Group B Streptococcus, potentially reducing neonatal complications.