Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fermentation, isolation, characterization and structure of nitrosofungin.

L A Dolak, T M Castle, B R Hannon

    The Journal of Antibiotics
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Carbon-carbon-linked (pyrazolylphenyl)oxazolidinones with antibacterial activity against multiple drug resistant gram-positive and fastidious gram-negative bacteria.

    Bioorganic & medicinal chemistry·2001
    Same author

    Identification of novel potent hydroxamic acid inhibitors of peptidyl deformylase and the importance of the hydroxamic acid functionality on inhibition.

    Bioorganic & medicinal chemistry letters·2001
    Same author

    Tipranavir (PNU-140690): a potent, orally bioavailable nonpeptidic HIV protease inhibitor of the 5,6-dihydro-4-hydroxy-2-pyrone sulfonamide class.

    Journal of medicinal chemistry·1998
    Same author

    (-)-6-Chloro-2-[(1-furo[2, 3-c]pyridin-5-ylethyl)thio]-4-pyrimidinamine, PNU-142721, a new broad spectrum HIV-1 non-nucleoside reverse transcriptase inhibitor.

    Journal of medicinal chemistry·1998
    Same author

    Structure-based design of nonpeptidic HIV protease inhibitors: the sulfonamide-substituted cyclooctylpyramones.

    Journal of medicinal chemistry·1997
    Same author

    Synthesis and biological activities of (R)-5,6-dihydro-N,N-dimethyl-4H-imidazo[4,5,1-ij]quinolin-5-amine and its metabolites.

    Journal of medicinal chemistry·1997
    Same journal

    Macrolide antibiotic action, resistance, and tolerance, and approaches to improve their activity against Gram-negative bacteria.

    The Journal of antibiotics·2026
    Same journal

    Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells.

    The Journal of antibiotics·2026
    Same journal

    Biosynthesis and enzymology of azaindane natural products.

    The Journal of antibiotics·2026
    Same journal

    Inhibition of Proteus mirabilis growth through gallium-based disruption of iron and heme metabolism.

    The Journal of antibiotics·2026
    Same journal

    Artificial intelligence-based phenotypic screening using Aspergillus oryzae identified a novel antifungal RK-1205-I.

    The Journal of antibiotics·2026
    Same journal

    Catenulamide A, an acylated tripeptide containing 5-methoxy-L-kynurenine lactam from an actinomycete of the genus Catenuloplanes.

    The Journal of antibiotics·2026
    See all related articles

    A novel antifungal compound, nitrosofungin, was discovered from a bacterial and Streptomyces co-culture. This agent shows broad-spectrum antifungal activity and low toxicity in animal models, offering a promising new therapeutic option.

    Area of Science:

    • Microbiology
    • Natural Product Chemistry
    • Pharmacology

    Background:

    • Fungal infections pose a significant global health challenge.
    • Development of new antifungal agents is crucial due to rising resistance.
    • Microbial sources are a rich resource for novel bioactive compounds.

    Purpose of the Study:

    • To isolate and characterize a new antifungal agent from a microbial co-culture.
    • To evaluate the in vitro antifungal spectrum and in vivo toxicity of the novel compound.
    • To elucidate the chemical structure and properties of the identified agent.

    Main Methods:

    • Co-cultivation of Alcaligenes (UC 9152) and Streptomyces plicatus (UC 8272).
    • Isolation and purification of the antifungal compound nitrosofungin.

    Related Experiment Videos

  • In vitro antifungal susceptibility testing against pathogenic fungi.
  • Toxicology studies in small laboratory animals.
  • Chemical identification and characterization of nitrosofungin.

    • Main Results:

    • Nitrosofungin was isolated in high yields from the mixed culture.
    • The bacterium produces the agent, while the Streptomyces enhances its production.
    • Nitrosofungin demonstrated broad-spectrum inhibition of pathogenic fungi in vitro.
    • The compound exhibited low toxicity in animal models with good systemic and oral bioavailability.
    • Nitrosofungin was identified as 2-N-nitrosohydroxylamino-1-propanol, a water-soluble N-nitrosohydroxylamine.

    Conclusions:

    • Nitrosofungin is a novel antifungal agent produced by a bacterial-Streptomyces co-culture.
    • Its broad-spectrum activity and favorable toxicity profile suggest therapeutic potential.
    • Further research into nitrosofungin's mechanism of action and clinical efficacy is warranted.