A rapid enzyme-immunoassay for the detection of ciguatoxin in contaminated fish tissues

An enzyme-immunoassay procedure for the detection of ciguatoxin has been developed using the sheep anti-ciguatoxin serum described earlier in the radioimmunoassay method for ciguatoxin. In the enzyme-immunoassay procedure, the sheep anti-ciguatoxin was coupled to horseradish peroxidase. Analyses of fish tissues showed that the enzyme-immunoassay procedure distinguished between clinically documented toxic and nontoxic tissues (P less than 0.001). Comparisons of the enzyme-immunoassay method with the radioimmunoassay for ciguatoxin and with a mouse bioassay demonstrated significant associations (P less than 0.001 and P less than 0.01, respectively). Preliminary studies showed that purified ciguatoxin inhibited the binding of sheep anti-ciguatoxin horseradish peroxidase to toxic fish tissues. Results suggest that the enzyme-immunoassay procedure may be valuable for routine direct assessment of ciguatoxin in fish tissues because of its practicality, sensitivity and specificity.