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A computerised enzyme immunosensor: application for the determination of antigens
Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 16, 1984
Summary
A novel enzyme immunosensor offers a sensitive amperometric determination of antigens in serum, providing a faster, isotope-free alternative to traditional immunochemical techniques for hepatitis B surface antigen antibodies.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunosensors
Background:
- Traditional immunochemical techniques for antigen determination often involve isotopes and are time-consuming.
- There is a need for faster, more convenient, and sensitive methods for antigen quantification in serum.
Purpose of the Study:
- To present preliminary results of a new sensitive amperometric method for antigen determination.
- To develop an enzyme immunosensor as a viable alternative to radioimmunoassay.
Main Methods:
- Utilized specific antibodies immobilized on a gelatin membrane in a solid-phase sandwich assay.
- Antibodies were labeled with glucose oxidase, and the enzymatic reaction was measured using a pO2 electrode.
- A computerized system recorded the oxygen consumption signal correlated to antigen concentration.
Main Results:
- Preliminary results demonstrate a sensitive amperometric determination of antigens.
- The method is less time-consuming and avoids the inconveniences of isotope use.
- The enzyme immunosensor signal is correlated to antigen concentration based on diffusional and reactional characteristics.
Conclusions:
- The developed enzyme immunosensor is a promising tool for sensitive amperometric determination of antigens in serum.
- This method offers advantages in speed and convenience over existing techniques.
- The technology has potential for broad application in assaying various antigens and haptens.