Alkali-extraction of membranes from mouse erythrocytes infected with Plasmodium berghei

Alkali-extracted membrane material from hypotonically lysed Plasmodium berghei-infected mouse erythrocytes has been analyzed by dodecyl sulphate polyacrylamide gel electrophoresis and fractionated by discontinuous sucrose gradient centrifugation. Components characteristic for the protein pattern associated with P. berghei infection have been recovered in the alkali-extracted material. Parasite components, free of host cell membrane contamination, have been obtained as a high-density fraction from sucrose gradients. This fraction is characterized by polypeptides with apparent molecular weight of 71,000 and 52,000.