Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The cell surface and its metabolism.

M J Crumpton, R J Owens, C J Gallagher

    The Journal of Pathology
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of the LDBio ICT IgG/IgM lateral flow assay versus the Bordier Elisa assay for the diagnosis of chronic pulmonary aspergillosis in Nigeria.

    Microbiology spectrum·2025
    Same author

    Schistosoma mansoni cathepsin D1: Biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells.

    Protein expression and purification·2019
    Same author

    OV21/PETROC: a randomized Gynecologic Cancer Intergroup phase II study of intraperitoneal versus intravenous chemotherapy following neoadjuvant chemotherapy and optimal debulking surgery in epithelial ovarian cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2017
    Same author

    Biochemistry of major human histocompatibility antigens.

    Immunology today·2014
    Same author

    Vitamin D: do we get enough? A discussion between vitamin D experts in order to make a step towards the harmonisation of dietary reference intakes for vitamin D across Europe.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2012
    Same author

    Migration and health service delivery.

    World hospitals and health services : the official journal of the International Hospital Federation·2010
    Same journal

    Knowledge gaps in tubular gut tumours: a critical appraisal of the 6th edition of the World Health Organization classification of tumours.

    The Journal of pathology·2026
    Same journal

    Tumor Invasive Border Index (TIBI) in colorectal cancer: linking infiltrative morphology to molecular insights.

    The Journal of pathology·2026
    Same journal

    Lipodystrophy and adipose tissue recovery are mediated by the Wnt/lipogenesis axis during skin fibrosis.

    The Journal of pathology·2026
    Same journal

    Optical mapping reveals a higher level of large-scale structural variants in a family with paternally transmitted myotonic dystrophy and independent Parkinson's disease.

    The Journal of pathology·2026
    Same journal

    FTO-mediated m6A modification of protein disulfide-isomerase activates VEGFA-VEGFR2 to suppress programmed cell death in osteosarcoma.

    The Journal of pathology·2026
    Same journal

    Dopamine inhibits retinal pathological neovascularization in the oxygen-induced retinopathy mouse model.

    The Journal of pathology·2026
    See all related articles

    Cell surface receptors dynamically change, influenced by cytoskeleton interactions. A specific 68,000-Mr protein

    Area of Science:

    • Cell Biology
    • Immunology
    • Biochemistry

    Background:

    • Cell surface receptor dynamics, including ligand binding, redistribution, and internalization, are crucial for cellular signaling and responsiveness.
    • Intracellular cytoskeleton components, particularly sub-membranous filamentous networks, are implicated in regulating cell surface structure modulation.
    • Erythrocyte plasma membranes provide a model for isolating detergent-insoluble cytoskeletal networks.

    Purpose of the Study:

    • To investigate the detergent-insoluble cytoskeletal components associated with the plasma membrane of human B lymphoblastoid cells.
    • To characterize the protein composition of this sub-membranous network and identify key molecular players.
    • To compare the cytoskeletal associations in lymphoblastoid cells with those in Burkitt lymphoma cells to understand potential differences related to malignancy.

    Related Experiment Videos

    Main Methods:

    • Isolation of plasma membrane fractions from human B lymphoblastoid cells.
    • Extraction of detergent-insoluble residues using nonionic detergents.
    • Analysis of protein composition in the detergent-insoluble fraction using gel electrophoresis and molecular weight determination.

    Main Results:

    • A detergent-insoluble residue was obtained from lymphoblastoid cell plasma membranes, containing actin and a 68,000-Mr polypeptide as major components.
    • Other prominent polypeptides (28,000-, 33,000-, and 120,000-Mr) were also identified in the insoluble fraction.
    • The association of the 68,000-Mr protein with the plasma membrane was calcium (Ca2+)-dependent, and this protein was notably absent from the inner face of Burkitt lymphoma cell membranes.

    Conclusions:

    • Human B lymphoblastoid cells possess a calcium-dependent, detergent-insoluble cytoskeletal network at the cytoplasmic face of their plasma membrane, involving actin and a 68,000-Mr protein.
    • The absence of this 68,000-Mr protein association in Burkitt lymphoma cells suggests a potential difference linked to the malignant phenotype or a specific B lymphocyte sub-population.
    • These findings highlight the role of specific cytoskeletal proteins in regulating cell surface dynamics and offer insights into potential molecular distinctions between normal and malignant B cells.