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Related Experiment Videos

Alcohol dehydrogenase activity in the developing chick embryo.

R W Wilson, G W Kalmus, S N Pennington

    Comparative Biochemistry and Physiology. B, Comparative Biochemistry
    |January 1, 1984
    PubMed
    Summary

    Chick embryos develop alcohol dehydrogenase (ADH) activity after day 9 of incubation, with ethanol clearance improving by day 13. Ethanol exposure does not alter ADH development or isozyme patterns.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Pharmacology

    Background:

    • Alcohol dehydrogenase (ADH) is crucial for ethanol metabolism.
    • Understanding ADH development in avian embryos provides insights into metabolic pathways.
    • Early life exposure to ethanol can impact developmental processes.

    Purpose of the Study:

    • To characterize the developmental profile of alcohol dehydrogenase (ADH) activity and isozymes in chick embryos.
    • To investigate the impact of early ethanol exposure on ADH development.
    • To examine changes in ADH kinetic properties during embryonic development.

    Main Methods:

    • Assessing ADH activity in chick embryo livers at various incubation days.
    • Analyzing ADH isozyme patterns using electrophoresis.

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  • Measuring kinetic parameters (Km apparent) of ADH.
  • Administering ethanol at the start of incubation and monitoring its clearance.

    • Main Results:

    • Chick embryo livers show no measurable ADH activity before day 9 of incubation.
    • ADH activity increases linearly with liver mass after day 9.
    • Two ADH isozymes are consistently detected, with stable relative contributions.
    • Ethanol is cleared slowly before day 9 but completely by day 13.
    • Apparent Km of ADH increases significantly post-hatching.
    • Prenatal ethanol exposure did not affect ADH activity or isozyme distribution.

    Conclusions:

    • Chick embryo liver ADH activity and kinetic properties undergo significant developmental changes.
    • The developing chick embryo exhibits distinct ethanol clearance capabilities.
    • Early ethanol exposure does not appear to disrupt the normal development of ADH in chick embryos.