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A quantitative microassay for leukocyte chemotaxis, using a microscopic slide system with complement-activating yeast

B I Coble, C Dahlgren, J Hed

    Journal of Immunological Methods
    |November 25, 1983
    PubMed
    Summary

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    This study introduces a new microassay for quantifying polymorphonuclear leukocyte (PMNL) chemotaxis, especially when cell numbers are limited. The assay measures PMNL migration towards a chemotactic gradient formed by yeast particles and complement activation.

    Area of Science:

    • Immunology
    • Cell Biology
    • Microbiology

    Background:

    • Polymorphonuclear leukocytes (PMNLs) are crucial for immune responses.
    • Assessing PMNL chemotaxis is vital for understanding immune function.
    • Existing methods can be limited by cell availability, particularly in clinical samples.

    Purpose of the Study:

    • To develop a simple, quantitative microassay for PMNL chemotaxis.
    • To enable chemotaxis studies when cell numbers are a limiting factor.
    • To provide a reliable method for analyzing PMNL migration in challenging sample types.

    Main Methods:

    • A microassay using glass slides with fixed, fluorescein-labeled yeast particles.
    • Adherent PMNLs are exposed to normal human serum to activate complement.

    Related Experiment Videos

  • Chemotaxis is quantified by scoring PMNL-associated yeast particles.
  • A locomotory index is calculated based on PMNL-yeast particle association.

    • Main Results:

    • The assay successfully quantifies PMNL chemotaxis.
    • It is effective even with limited numbers of PMNLs.
    • The method allows for comparative analysis against control conditions (inactivated serum or no serum).

    Conclusions:

    • A novel and simple quantitative microassay for PMNL chemotaxis has been established.
    • This method is suitable for situations with limited cell availability, such as in clinical settings.
    • The developed assay facilitates the study of PMNL function in various biological samples.