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[Incompatibility properties of pKMR plasmids determining antibiotic resistance and the capacity to produce
Abstract:
pKMR207-1, pKMR208-1, pKMR209, pKMR210 and pKMR212 plasmids controlling simultaneously antibiotic resistance and capacity for synthesis of the colonization antigen were detected in wild strains of E. coli of various serotypes. The properties of compatibility of plasmids pKMR to each other and their attitude to the known incompatibility groups were studied. It was shown that pKMR207-1, pKMR208-1, pKMR210 and pKMR212 plasmids were incompatible. These plasmids had the incompatibility properties with respect to R388 (Inc FI) plasmid and probably belonged to the incompatibility group Inc. FI. pKMR209 plasmid was compatible with pKMR207-1, pKMR208-1, pKMR210 and pKMR212 plasmids and belonged to another incompatibility group.
Insights
New plasmids in wild E. coli strains confer antibiotic resistance and colonization antigen synthesis. Researchers studied plasmid compatibility, finding some E. coli plasmids are incompatible with each other and belong to the Inc. FI group.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Wild E. coli strains can harbor plasmids that confer advantageous traits.
- Antibiotic resistance and the ability to synthesize colonization antigens are significant factors in bacterial virulence and persistence.
Purpose of the Study:
- To identify and characterize plasmids in wild E. coli strains.
- To investigate the compatibility properties of these plasmids with each other and known incompatibility groups.
Main Methods:
- Detection of plasmids in wild E. coli strains.
- Compatibility testing of identified plasmids.
- Classification of plasmids into incompatibility groups.
Main Results:
- Five plasmids (pKMR207-1, pKMR208-1, pKMR209, pKMR210, and pKMR212) were identified in wild E. coli, conferring antibiotic resistance and colonization antigen synthesis.
- Plasmids pKMR207-1, pKMR208-1, pKMR210, and pKMR212 were found to be incompatible with each other and with the R388 plasmid (Inc FI), suggesting they belong to the Inc FI group.
- Plasmid pKMR209 was compatible with the other pKMR plasmids, indicating it belongs to a different incompatibility group.
Conclusions:
- Wild E. coli strains harbor diverse plasmids with significant implications for antibiotic resistance and pathogenicity.
- The characterization of pKMR plasmids provides insights into plasmid evolution and epidemiology within E. coli populations.
- Understanding plasmid incompatibility is crucial for predicting co-existence and transfer of antibiotic resistance genes.