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Extractable immune complex in soluble substances from psoriatic scales.
Archives of Dermatological Research
|January 1, 1984
Summary
Researchers identified immune complexes (IC) in psoriatic scales, suggesting they play a direct role in psoriasis pathogenesis. These findings indicate ICs, composed of IgG-IgA, bind epidermal proteins and are integral to skin lesion immune reactions.
Area of Science:
- Immunodermatology
- Molecular immunology
- Psoriasis research
Background:
- Psoriatic lesions exhibit deposits of immunoglobulins and complement.
- The precise role of these deposits in psoriasis pathogenesis remains unclear.
Purpose of the Study:
- To investigate the presence and characteristics of immune complexes (IC) in soluble extracts of psoriatic scales.
- To compare immune reactivity in psoriatic scales with those from pityriasis rubra pilaris.
Main Methods:
- Immunoelectrophoresis to detect IgG, IgA, and C3.
- Sephadex G-200 column chromatography for molecular weight analysis.
- Raji cell assay and anti-IgG affinity chromatography to characterize immune complexes.
Main Results:
- Psoriatic scale extracts contained IgG, IgA, and C3, while pityriasis rubra pilaris extracts only showed IgG.
- High molecular weight IgG and IgA fractions (>200,000 daltons) were identified in psoriatic extracts.
- These fractions demonstrated immune complex characteristics, specifically IgG-IgA complexes, binding epidermal proteins.
Conclusions:
- Immune complexes (IC) composed of IgG-IgA are present in psoriatic scales.
- These ICs appear to bind epidermal proteins, suggesting a direct role in psoriatic lesion immune reactions.
- The findings challenge the notion that immunoglobulin deposits are merely secondary to dermal inflammation.