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Antimonocyte serum. Preparation and characterization.

T Gościcka, W Rudnicka, M Walencka

    Archivum Immunologiae Et Therapiae Experimentalis
    |January 1, 1983
    PubMed
    Summary
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    Researchers developed specific antisera for monocytes using pig and rabbit blood. These antisera, rabbit anti-monocyte serum (RAMS) and sheep anti-monocyte serum (SAMS), were purified by removing antibodies against lymphocytes and granulocytes.

    Area of Science:

    • Immunology
    • Cell Biology
    • Veterinary Science

    Background:

    • Monocytes are crucial immune cells involved in phagocytosis and antigen presentation.
    • Listeria monocytogenes is a bacterial pathogen that can infect various hosts.
    • Developing specific antibodies against cell types is essential for immunological research.

    Purpose of the Study:

    • To generate specific antisera against monocytes from pigs and rabbits.
    • To characterize these antisera for their specificity towards monocytes.

    Main Methods:

    • Cell suspensions enriched with monocytes were isolated using BSA and Ficoll-Triosil density gradients.
    • Rabbits and a sheep were immunized with these monocyte-enriched cells.
    • Antisera were rigorously absorbed with lymphocytes and granulocytes to ensure monocyte specificity.

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    Main Results:

    • Rabbit anti-monocyte serum (RAMS) and sheep anti-monocyte serum (SAMS) were successfully produced.
    • Absorption procedures effectively removed antibodies against lymphocytes and granulocytes, yielding specific monocyte antisera.

    Conclusions:

    • Specific antisera (RAMS and SAMS) against monocytes were successfully generated and purified.
    • These reagents are valuable tools for further immunological studies involving monocytes in pigs and rabbits.