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[Plasmid pAP20 controlling the hemolytic activity of E. coli]
Biulleten' Eksperimental'Noi Biologii I Meditsiny
|March 1, 1984
Abstract:
The authors investigated pAP20 plasmid identified in E. coli cells isolated from man. According to the evidence obtained pAP20 plasmid determines the synthesis of alpha-hemolysin, being an F-like plasmid of the drd type. Having medium molecular size, the plasmid belongs to the inc FIV group and is partly incompatible with pAP38 plasmid which is a reference plasmid of the inc FVII group.
Insights
The pAP20 plasmid in human E. coli synthesizes alpha-hemolysin. This F-like plasmid is classified under inc FIV and shows incompatibility with inc FVII plasmids.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Escherichia coli (E. coli) are common bacteria found in humans.
- Plasmids are extrachromosomal DNA elements that can carry genes conferring advantageous traits.
- Alpha-hemolysin is a toxin produced by some E. coli strains.
Purpose of the Study:
- To characterize the pAP20 plasmid found in human E. coli.
- To determine the function and genetic properties of the pAP20 plasmid.
Main Methods:
- Isolation and identification of E. coli from human samples.
- Plasmid DNA analysis.
- Genetic characterization of plasmid-borne genes.
Main Results:
- The pAP20 plasmid was identified in E. coli from human sources.
- pAP20 determines the synthesis of alpha-hemolysin.
- pAP20 is an F-like plasmid of the drd type.
- It belongs to the inc FIV group and has medium molecular size.
- Partial incompatibility was observed with pAP38 (inc FVII).
Conclusions:
- The pAP20 plasmid is a significant genetic element in human E. coli, responsible for alpha-hemolysin production.
- Its classification as inc FIV and partial incompatibility with inc FVII provide insights into plasmid evolution and epidemiology.