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Male-specific transplantation antigen expression by XY teratocarcinomas PCC7 and 7'
Abstract:
Male-specific antigen expression by XY teratocarcinomas PCC7 and 7' is demonstrated first by the rejection of tumors by female but not by male mice following challenge with these cell lines. Male-specific antigen expression is confirmed by an indirect method in which females are immunized against H-Y antigen by male skin grafts. A variant of PCC7 lacking male-specific antigen expression is described. Analysis of the karyotype and of the DNA from this variant indicate that the loss of male-specific antigen expression is a result of the loss of the Y chromosome. The ability to recover variants that have lost expression of male-specific antigen opens the possibility of their selection after mutagenesis.
Insights
XY teratocarcinomas (PCC7 and 7') express male-specific antigens, leading to tumor rejection in female mice but not male mice. Loss of the Y chromosome in a PCC7 variant eliminated this antigen expression.
Area of Science:
- Immunology
- Developmental Biology
- Cancer Research
Background:
- Teratocarcinomas are tumors with diverse cell types.
- XY teratocarcinomas can express male-specific antigens.
- H-Y antigen plays a role in immune responses.
Purpose of the Study:
- To demonstrate male-specific antigen expression in XY teratocarcinomas.
- To investigate the genetic basis of male-specific antigen expression.
- To explore the potential for selecting antigen-loss variants.
Main Methods:
- Tumor challenge experiments in male and female mice.
- Indirect immunization assays using H-Y antigen.
- Karyotype and DNA analysis of teratocarcinoma variants.
Main Results:
- PCC7 and 7' teratocarcinomas showed rejection in female mice, indicating male-specific antigen expression.
- A PCC7 variant lacking male-specific antigen expression was identified.
- Loss of the Y chromosome correlated with the absence of male-specific antigen expression.
Conclusions:
- XY teratocarcinomas express male-specific antigens.
- The Y chromosome is essential for male-specific antigen expression in these cells.
- The identification of antigen-loss variants facilitates further research, including mutagenesis studies.
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