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Related Experiment Videos

Complement factor D concentrations in normal sera: comparison of immunochemical and functional determinations.

G Sturfelt, L Truedsson

    Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology
    |December 1, 1983
    PubMed
    Summary
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    Factor D (D) levels were measured in healthy adults and children. Concentrations of D were found to be lower in children compared to adults, with good correlation between two measurement methods.

    Area of Science:

    • Biochemistry
    • Immunology

    Background:

    • Factor D (D) is a key component of the complement system.
    • Accurate measurement of Factor D concentration is important for understanding its role in health and disease.

    Purpose of the Study:

    • To determine the concentration of Factor D in healthy adults and children.
    • To compare two different assay methods for measuring Factor D levels.
    • To establish reference ranges for Factor D in different age groups.

    Main Methods:

    • Enzyme amplified electroimmunoassay (EAE) and hemolysis in gel (HIG) assay were used to quantify Factor D.
    • Sera from 144 healthy adults and 29 healthy children were analyzed.
    • Results were correlated between the two assay methods.

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    Main Results:

    • Established 95% geometric confidence ranges for Factor D in adults: 75-143% (EAE) and 65-171% (HIG).
    • Factor D levels were significantly lower in children than in adults.
    • A moderate positive correlation (r = 0.52, p < 0.001) was observed between EAE and HIG assay results.

    Conclusions:

    • The study provides reference ranges for Factor D in healthy populations.
    • Factor D levels differ between adult and pediatric age groups.
    • Both EAE and HIG assays are reliable methods for Factor D quantification, showing good correlation.