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Updated: Jul 12, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
Comparative mutagenesis by aminofluorene derivatives. A possible effect of DNA configuration
Abstract:
The mutagenicity of N-acetoxy-N-2-acetylaminofluorene (N-acetoxy- 2AAF ) for Salmonella typhimuricum TA98 is greatly reduced when compared to that of N-hydroxy-2-aminofluorene. This decrease in mutagenic response is accompanied by the formation of a deoxyguanosine-2-acetylaminofluorene adduct. The deoxyguanosine-2-aminofluorene adduct, characteristic of cells exposed to N - hydroxy-2-aminofluorene, was not detected in N-acetoxy- 2AAF -treated cells. Enzymic deacetylation of N - acetoxy- 2AAF results in restoration of potent mutagenicity. N-Acetoxy-2-acetylamino-7- iodofluorene is also more mutagenic than N-acetoxy- 2AAF . Because the acetylated and unacetylated guanine adducts induce greatly different configurational changes, the results may be indicative that the introduction of the syn configuration and a possible shift to the Z-conformation at the mutational hot spot of Salmonella typhimurium TA98 [(dG-dC)8] results in reduced mutagenic potency.
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