[Determination of cell wall amino sugars in Streptococcus on a carbohydrate analyzer]

A method of determining aminosaccharides (muramic acid, glucosamine and galactosamine) by means of a carbohydrate analyzer "Biotronic" using the cation-exchange resin DC-6 A ("Durrum") was developed. Chromatographic conditions correspond to the conditions of neutral sugar analysis on a column with DCh-4 resin, that enables after a slight modification of the analyzer to pass from the determining of aminosaccharides to the determining of neutral sugars. The method was used for determining the carbohydrate composition of streptococcus cell walls. The results obtained allow to conclude that using this method one can get more information on the hydrocarbon composition of various biological objects than using the method of aminosaccharide determining by means of aminoacid anylyzer, which is widely in practice nowadays.