Embedding, cutting and grinding methods to produce undecalcified cortical bone sections over the whole bone

Polyethylene bags as a container material are flexible and elastic enough to follow the shrinkage of monomers during the polymerization process, since the main cause of bubble building in the polymerization material is the shrinkage of the monomer methacrylic acid methylester. The polymerization time in polyethylene containers is shorter (3-5 days) than in glass containers (1-2 weeks). The length of the bone and the thickness of the cutting bone sections are the limiting factors for two different cutting and grinding procedures, which were described.