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Isolation of the ST Ent plasmid specific bacteriophage M124

P Soedarmono, A Matsushiro, K Hoshi

    Biken Journal
    |September 1, 1983
    PubMed

    Insights

    Researchers isolated phage M124 for simple detection of enterotoxigenic E. coli. This bacteriophage specifically targets E. coli with the ST Ent plasmid, aiding in identifying ST enterotoxigenic bacteria.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Virology

    Background:

    • Enterotoxigenic Escherichia coli (E. coli) are significant human pathogens.
    • Accurate and rapid detection methods for these bacteria are crucial for public health.
    • Current detection methods may require complex laboratory procedures.

    Purpose of the Study:

    • To isolate and characterize a bacteriophage capable of specifically detecting enterotoxigenic E. coli.
    • To evaluate the utility of the isolated bacteriophage for clinical diagnostics.

    Main Methods:

    • Screening for bacteriophages that specifically lyse enterotoxigenic E. coli strains.
    • Isolation and identification of a specific bacteriophage (M124).
    • Analysis of plasmid DNA and detection of ST enterotoxin in E. coli strains targeted by the phage.

    Main Results:

    • Phage M124 was successfully isolated.
    • Phage M124 demonstrated specific proliferation on E. coli strains carrying the 80 Mdalton ST Ent plasmid.
    • The phage specifically attacks E. coli harboring the ST Ent plasmid.

    Conclusions:

    • Phage M124 is a highly specific bacteriophage for detecting ST enterotoxigenic E. coli.
    • This phage offers a promising tool for simple and rapid detection of these pathogenic bacteria.
    • Phage M124 can be a valuable asset in clinical microbiology and diagnostics.

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