[Study of low temperature crystallization in E. coli cells using NMR]

Freezing and thawing processes of the E. coli cell suspension have been studied by NMR. It was shown that the degree of the cell dehydration correlated with its freezing time. The effect of the recrystallization processes was evaluated and its temperature range was indicated. It was noted that nonfreezing water content increased during thawing of the cells as compared to this content at the same temperature during freezing.