Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synthetic peptide substrates in hemostatic testing.

J Fareed, H L Messmore, J M Walenga

    Critical Reviews in Clinical Laboratory Sciences
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pharmacological profile of sulodexide.

    International angiology : a journal of the International Union of Angiology·2014
    Same author

    Medical management of patients with peripheral arterial disease.

    International angiology : a journal of the International Union of Angiology·2014
    Same author

    Prevention and treatment of venous thromboembolism--International Consensus Statement.

    International angiology : a journal of the International Union of Angiology·2014
    Same author

    Association between serum levels of pro-metalloproteinase 1, tissue inhibitor of metalloproteinases 1 and 2 and prevalent cardiovascular disease in a population-based study.

    International angiology : a journal of the International Union of Angiology·2013
    Same author

    Update of the recommendations on biosimilar low-molecular-weight heparins from the Scientific Subcommittee on Control of Anticoagulation of the International Society on Thrombosis and Haemostasis.

    Journal of thrombosis and haemostasis : JTH·2013
    Same author

    Thromboelastographic evaluation of blood coagulation in the presence of branded and generic enoxaparins.

    International angiology : a journal of the International Union of Angiology·2012

    Synthetic substrates revolutionize coagulation testing, offering improved functional assays over immunological methods for factors like antithrombin-III and heparin. These advanced methods better reflect clinical relevance in thrombotic disorders.

    Area of Science:

    • Biochemistry
    • Hematology
    • Clinical Chemistry

    Background:

    • Traditional coagulation testing has limitations in assessing functional protein activity.
    • Synthetic chromogenic and fluorogenic peptide substrates offer new methodologies.
    • Coagulation profiling and automation are emerging concepts.

    Purpose of the Study:

    • To evaluate the advancements in coagulation testing using synthetic peptide substrates.
    • To compare the clinical relevance of functional assays versus immunological methods.
    • To explore the application of these methods in assessing various coagulation factors and inhibitors.

    Main Methods:

    • Development of synthetic substrate assays for numerous coagulation factors and inhibitors (e.g., antithrombin-III, heparin).

    Related Experiment Videos

  • Comparison of functional assays with immunological methods (nephelometry, immunodiffusion, radioimmunoassay).
  • Utilized human and bovine thrombin and factor Xa with specific substrates.

    • Main Results:

    • Synthetic substrate assays provide functional evaluation, unlike immunological methods which measure total antigen levels.
    • Immunological methods for antithrombin-III are questionable due to inability to distinguish active forms.
    • Functional activity of serine protease inhibitors is often reduced in pathological samples, while immunological levels remain minimally changed.
    • Heparin concentration assays are satisfactory but do not reflect overall anticoagulant effect.
    • Fluorogenic substrates can detect activated coagulation factors in thrombotic disorders.

    Conclusions:

    • Synthetic substrate assays offer superior clinical relevance for coagulation testing compared to immunological methods.
    • Functional assays are crucial for accurate assessment of coagulation factor activity.
    • New methods enable better detection of activated factors in thrombotic conditions.