Related Experiment Videos
Alpha-actinin arcs in megakaryocyte spreading.
Experimental Cell Research
|June 1, 1984
Summary
Adenosine diphosphate (ADP) triggers guinea pig megakaryocyte spreading and adhesion by inducing cytoskeletal changes. Fibrous arcs, containing alpha-actinin, form transient attachment sites during this cell spreading process.
Area of Science:
- Cell Biology
- Hematology
Background:
- Megakaryocytes are crucial for platelet production.
- Cell spreading and adhesion are fundamental cellular processes.
Purpose of the Study:
- To investigate the effect of adenosine diphosphate (ADP) on megakaryocyte morphology and adhesion.
- To examine the cytoskeletal rearrangements and attachment sites involved in megakaryocyte spreading.
Main Methods:
- Cultured guinea pig bone marrow megakaryocytes were treated with ADP.
- Transmission electron microscopy (TEM) and interference reflection microscopy were used.
- Immunofluorescence staining for alpha-actinin was performed.
Main Results:
- ADP induced megakaryocytes to spread and adhere to the substratum.
- Spreading involved the assembly of microfilaments into nets and bundles.
- Arc-shaped attachment regions containing alpha-actinin were observed, some corresponding to transient attachment sites.
Conclusions:
- ADP-induced megakaryocyte spreading involves significant cytoskeletal reorganization.
- Fibrous arcs containing alpha-actinin are involved in transient cell-substratum attachment.
- Similar structures may be common in other cell types during spreading.