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Carp preproinsulin cDNA sequence and evolution of insulin genes
Folia Biologica
|January 1, 1984
Summary
The carp preproinsulin cDNA sequence was determined, revealing its complete genetic code. This finding confirms carp insulin
Area of Science:
- Molecular Biology
- Genomics
- Endocrinology
Background:
- Insulin is a vital hormone regulating blood glucose.
- Understanding insulin gene evolution provides insights into metabolic regulation.
Purpose of the Study:
- To determine the nucleic acid sequence of carp (Cyprinus carpio) preproinsulin cDNA.
- To analyze the genetic information and evolutionary implications of carp preproinsulin.
Main Methods:
- Cloning of carp preproinsulin cDNA into pBR322.
- DNA sequencing of the cloned insert (439 bp).
- Bioinformatic analysis and comparison with other species.
Main Results:
- The complete coding sequence for carp preproinsulin (108 amino acids) was identified.
- The sequence includes 5' and 3' non-translated regions.
- Confirmed carp insulin amino acid sequence and determined signal peptide (21 amino acids) and C-peptide (35 amino acids).
- Observed a short signal peptide and an extended B chain, suggesting a shifted signal peptidase cleavage site.
Conclusions:
- The carp preproinsulin cDNA sequence provides a detailed genetic blueprint.
- Evolutionary analysis indicates a significant divergence between freshwater and saltwater teleosts, comparable to that between mammals and birds.