Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ames mutagenicity tests on purified 3-nitropropionic acid.

T J Hansen

    Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

    Physical review letters·2022
    Same author

    Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

    Physical review letters·2021
    Same author

    GW190521: A Binary Black Hole Merger with a Total Mass of 150  M_{⊙}.

    Physical review letters·2020
    Same author

    Chromosome aberrations in pressure-induced triploid Atlantic salmon.

    BMC genetics·2020
    Same author

    Effects of laboratory salmon louse infection on Arctic char osmoregulation, growth and survival.

    Conservation physiology·2019
    Same author

    Search for Subsolar Mass Ultracompact Binaries in Advanced LIGO's Second Observing Run.

    Physical review letters·2019

    3-Nitropropionic acid

    Area of Science:

    • Food chemistry
    • Toxicology
    • Microbiology

    Background:

    • 3-Nitropropionic acid (3-NPA) is a toxic mold metabolite found in fermented and spoiled foods.
    • Previous studies suggested 3-NPA is mutagenic to specific Salmonella typhimurium strains.

    Purpose of the Study:

    • To investigate the mutagenicity of 3-nitropropionic acid.
    • To determine if impurities in 3-NPA samples contributed to reported mutagenicity.

    Main Methods:

    • Bacterial mutagenicity testing using Salmonella typhimurium strains (TA98, TA100, TA1538).
    • Testing was performed with and without metabolic activation.
    • Mutagenicity assays were conducted before and after recrystallization of 3-NPA samples.

    Main Results:

    Related Experiment Videos

    • An impure 3-NPA sample showed mutagenicity in Salmonella strain TA100, which decreased after purification.
    • The impure sample was not mutagenic in strain TA98.
    • A purer 3-NPA sample was non-mutagenic across all tested Salmonella strains.

    Conclusions:

    • The previously reported mutagenicity of 3-nitropropionic acid was likely due to impurities, not the compound itself.
    • Purification significantly reduced or eliminated the mutagenic potential of 3-NPA samples.
    • This highlights the importance of sample purity in toxicological assessments.