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The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3 cells are induced to synthesize light chain and become high level membrane IgM expressors . The cytokine (s) responsible for inducing 70Z /3 cells to differentiate is different from interleukin-2 (IL-2), B-cell growth factor (BCGF), colony-stimulating factor (CSF) I and II, but one of the cytokines is either very similar or identical to interleukin-1 (IL-1). These results demonstrate that IL-1 or a molecule with very similar physical properties can act directly on a B-cell line and thus probably also on normal B cells to influence differentiation.
The murine pre-B-like cell line, 70Z /3, has been shown to undergo differentiation in response to supernatants derived from concanavalin-A (Con A) stimulated rat spleen cells or ultraviolet light treated P388D1 macrophage cell line. After culture for 24 h with supernatant factors, normally membrane IgM negative 70Z /3 cells are induced to synthesize light chain and become high level membrane IgM expressors . The cytokine (s) responsible for inducing 70Z /3 cells to differentiate is different from interleukin-2 (IL-2), B-cell growth factor (BCGF), colony-stimulating factor (CSF) I and II, but one of the cytokines is either very similar or identical to interleukin-1 (IL-1). These results demonstrate that IL-1 or a molecule with very similar physical properties can act directly on a B-cell line and thus probably also on normal B cells to influence differentiation.