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Accessory function and interleukin 1 production by human leukemic cell lines.

H Wakasugi, A Harel, M C Dokhelar

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1984
    PubMed
    Summary
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    Human leukemic cell lines can replace monocytes in supporting T cell proliferation and producing interleukin 1 (IL 1). These findings highlight novel sources for IL 1 and suggest its production is not limited to the monocytic lineage.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • T lymphocyte proliferation requires accessory cells like monocytes.
    • Monocyte accessory function (AF) is mediated by interleukin 1 (IL 1).
    • The precise cellular requirements for IL 1 production and T cell support are not fully understood.

    Purpose of the Study:

    • To investigate the potential of human leukemic cell lines as substitutes for monocytes in T cell assays.
    • To explore the relationship between leukemic cell differentiation and accessory function.
    • To characterize the IL 1 produced by these cell lines and its functional properties.

    Main Methods:

    • Mitogen-induced T cell proliferation assays using purified T lymphocytes and leukemic cell lines (K562, HL60, U937).

    Related Experiment Videos

  • Induction of differentiation in leukemic cell lines using specific inducers.
  • Analysis of IL 1 production and characterization by gel filtration and isoelectric focusing.
  • Phenotypic marker analysis, including HLA-DR expression.

    • Main Results:

    • K562, HL60, and U937 cells efficiently replaced monocytes in supporting T cell proliferation.
    • Accessory function correlated with specific maturational stages of leukemic cells.
    • Induced differentiation of HL60 and U937 cells enhanced their accessory function.
    • Leukemic cells produced IL 1 indistinguishable from monocyte-derived IL 1.
    • IL 1 production and AF were observed in cell types lacking HLA-DR expression, decoupling these functions from monocytic lineage and HLA-DR presence.

    Conclusions:

    • Human leukemic cell lines are valuable tools for studying T cell accessory function and IL 1 production.
    • IL 1 production and T cell support are not restricted to the monocytic lineage and do not require HLA-DR expression.
    • These cell lines represent convenient and accessible sources of human IL 1 for research purposes.