Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polysaccharide storage in different streptococci.

C R McFarland, T L Snyder, R McKenzie

    Microbios
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A trial of nebulised heparin to limit lung injury following cardiac surgery.

    Anaesthesia and intensive care·2015
    Same author

    Safety and pharmacokinetics of XOMA 3AB, a novel mixture of three monoclonal antibodies against botulinum toxin A.

    Antimicrobial agents and chemotherapy·2014
    Same author

    Derrida, and all that jazz.

    Journal of psychiatric and mental health nursing·2012
    Same author

    Monte Carlo simulations of the electric field close to the body in realistic environments for application in personal radiofrequency dosimetry.

    Radiation protection dosimetry·2011
    Same author

    Plating media requirements for certain strains of Bacterium tularense.

    Journal of bacteriology·2010
    Same author

    A dilution plate counting method for certain strains of Bacterium tularense.

    Journal of bacteriology·2010
    Same journal

    Identification, cloning and expression of sodC from an alkaline phosphatase gene fusion library of Mycobacterium avium subspecies paratuberculosis.

    Microbios·2001
    Same journal

    Distribution of hepatitis C virus serotypes and duration of infection in HCV infected subjects from a geographical area in Southeast Italy.

    Microbios·2001
    Same journal

    Demonstration of genome DNA diversity of strains of urease-positive thermophilic Campylobacter isolated from the natural environment by pulsed-field gel electrophoresis analysis.

    Microbios·2001
    Same journal

    Toxic effects of HgCl2 on the growth and oogonium formation in Oedogonium hatei.

    Microbios·2001
    Same journal

    Growth inhibition of fouling diatoms by antipatharian colonies.

    Microbios·2001
    Same journal

    Inhibition by the essential oils of peppermint and spearmint of the growth of pathogenic bacteria.

    Microbios·2001
    See all related articles

    Group A Streptococcus requires specific medium supplements like polyvinylpyrollidones (PVP) and polyethylene glycols (PEG) for intracellular polysaccharide accumulation, which stabilizes the cell surface. This medium stabilization is unique to Group A Streptococcus strains and impacts polysaccharide storage.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Streptococcal Physiology

    Background:

    • Intracellular polysaccharide accumulation (IPS) is a characteristic of certain bacterial species.
    • Group A Streptococcus (GAS) synthesizes IPS, but the specific nutritional and environmental requirements are not fully understood.
    • Previous studies suggest medium components can influence bacterial physiology.

    Purpose of the Study:

    • To investigate the role of specific medium components in intracellular polysaccharide accumulation in Group A Streptococcus.
    • To determine if medium stabilization is a unique characteristic of GAS compared to other streptococci.
    • To explore factors influencing the degree of IPS storage in GAS.

    Main Methods:

    • Culturing various strains of Group A Streptococcus and other streptococci (cariogenic, S. agalactiae).

    Related Experiment Videos

  • Supplementing culture media with calf serum, pooled human plasma, polyvinylpyrollidones (PVP), and polyethylene glycols (PEG).
  • Assessing intracellular iodophilic polysaccharide (IPS) storage and evaluating the effects of medium components on cell surface stability.

    • Main Results:

    • Group A Streptococcus cultures required supplements like PVP and PEG for intracellular polysaccharide accumulation.
    • These supplements provided a stabilizing effect on the cell surface, not directly for nutrition during IPS storage.
    • Medium stabilization and maltose presence were unique to Group A Streptococcus; other streptococci showed stimulation but no requirement.

    Conclusions:

    • Medium stabilization via supplements like PVP and PEG is essential for IPS storage in many Group A Streptococcus strains.
    • This requirement for medium stabilization distinguishes Group A Streptococcus from cariogenic streptococci and S. agalactiae.
    • The extent of IPS storage in Group A Streptococcus is strain-dependent, likely influenced by the polymer's structure.