[Tetracycline hydrochloride complex formation with collagen]

The quantitative indices and regularities of tetracycline hydrochloride binding with colelagen in the process of complex formation were studied in vitro. It was found that tetracycline-interacted with collagen. The amount of the antibiotic bound depended on its initial concentration in the mixture and the aggregate state of the protein: the amount of tetracycline hydrochloride absorbed by dissloved collagen was 10 times higher than that absorbed by polymerized collagen. The antibiotic bound with collagen did not lose its specific antibacterial activity.