Related Experiment Video
Updated: Sep 22, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
[Relation between PBPs (penicillin-binding proteins) and growth curves of Escherichia coli submitted to ampicillin
Abstract:
Growth curves recorded by the MS-2 (high performance photometer) provide a new means for studying PBPs. Indeed, the increase in prelytic OD (observed during the first two hours of contact between beta- lactams and cultures) is concentration- independent for antibiotics bound to PBPs3 ( Yourassowsky et al., 1981). Oxacillin has high affinity for PBP3 of E. coli (Curtis et al., 1979). Growth curves confirm biochemical studies: prelytic increase of OD of E. coli cultures is concentration- independent for oxacillin (100-2000 micrograms/ml), conversely to results observed with ampicillin and amoxycillin.
Related Concept Videos
Antibiotic Selection
Stringent Response in E. coli
Production of Antibiotics
Inhibitors of Gram-positive Cell Wall Synthesis

