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Gentamicin/heparin interactions: effects on two immunoassays and on protein binding
Therapeutic Drug Monitoring
|January 1, 1984
Summary
Heparin affects gentamicin measurements, inhibiting enzyme immunoassays (EIA) at higher concentrations but not fluorescence polarization immunoassays (FPIA). Higher heparin levels also increase bound gentamicin fractions due to direct binding and complex interactions.
Area of Science:
- Pharmacology
- Clinical Chemistry
- Analytical Chemistry
Background:
- Heparin's effects on gentamicin quantitation are debated.
- Conflicting reports exist regarding heparin concentrations that influence gentamicin measurements.
Purpose of the Study:
- To investigate heparin's impact on gentamicin determination using enzyme immunoassay (EIA) and fluorescence polarization immunoassay (FPIA).
- To assess how varying heparin concentrations affect gentamicin binding and measurement accuracy.
Main Methods:
- Comparing gentamicin measurements by EIA and FPIA across different heparin concentrations (28 U/ml to 200 U/ml).
- Analyzing the effect of heparin on gentamicin distribution equilibrium by measuring free gentamicin in ultrafiltrates.
Main Results:
- Fluorescence polarization immunoassay (FPIA) showed no effect from heparin on gentamicin quantitation.
- Enzyme immunoassay (EIA) demonstrated significant inhibition (10-20%) of gentamicin determination at heparin concentrations of 100-200 U/ml.
- Increased heparin concentrations led to a higher bound fraction of gentamicin, suggesting direct binding and complex interactions.
Conclusions:
- Heparin interferes with gentamicin EIA at high concentrations, but not with FPIA.
- Heparin influences gentamicin's distribution, increasing its bound fraction through direct and complex interactions with proteins.